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cfx96 real time pcr detection system  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx96 real time pcr detection system
    Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+touch+real+time+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13091180-120-14-19
    Average 96 stars, based on 27901 article reviews
    cfx96 real time pcr detection system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: High sucralose exposure in early pregnancy increases the susceptibility of heart defects in mice offspring
    Article Snippet: Total RNA was extracted from mouse tissues or cultured cells by Trizol reagents (Cat#19211, Yeasen Biotechnology (Shanghai, China) Co., Ltd) and converted into cDNA using the HiScript III 1st strand cDNA Synthesis Kit (Cat#R312-02, Vazyme, Nanjing, China). .. Gata4 / 6 , Tbx1 / 3 / 5 / 20 , Nkx2.5 , Notch1 , Tas1r2 and Tas1r3 mRNA levels were measured by qRT-PCR using ChamQ SYBR Color qPCR Master Mix (Cat#Q311-02, Vazyme, Nanjing, China) on the CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, USA) with Actin as an internal reference gene. ..

    Article Title: Based on metabolome and transcriptome analysis, differences in organic acids of ‘Korla Xiangli’ fruit from different male parent
    Article Snippet: .. Gene-specific primers for the selected differentially expressed genes are listed in . qRT-PCR was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme). ..

    Real-time Polymerase Chain Reaction:

    Article Title: High sucralose exposure in early pregnancy increases the susceptibility of heart defects in mice offspring
    Article Snippet: Total RNA was extracted from mouse tissues or cultured cells by Trizol reagents (Cat#19211, Yeasen Biotechnology (Shanghai, China) Co., Ltd) and converted into cDNA using the HiScript III 1st strand cDNA Synthesis Kit (Cat#R312-02, Vazyme, Nanjing, China). .. Gata4 / 6 , Tbx1 / 3 / 5 / 20 , Nkx2.5 , Notch1 , Tas1r2 and Tas1r3 mRNA levels were measured by qRT-PCR using ChamQ SYBR Color qPCR Master Mix (Cat#Q311-02, Vazyme, Nanjing, China) on the CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, USA) with Actin as an internal reference gene. ..

    Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology
    Article Snippet: For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the iScript Select cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions. .. Real-time qPCR was performed in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using specific primers ( ) and the iQ SYBR Green Mix (Bio-Rad), according to the manufacturer’s protocol. ..

    Article Title: Real-time PCR to target Hoodia in herbal supplements: a tool for conservation and trade regulation
    Article Snippet: .. All real-time PCR runs were conducted using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, USA) and included a No Template Control (NTC). ..

    Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization
    Article Snippet: Total RNA was extracted using TRIpure RNA Isolation Reagent (TransGen Biotech) and reverse-transcribed using the 5× All-in-One RT Master Mix (ABM) according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT–PCR) was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using SYBR Green PCR Master Mix (ABclonal). ..

    Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming
    Article Snippet: After removing the culture supernatant, total RNA was extracted using TRIzol reagent (Takara, 9109). .. Reverse transcription was performed according to the manufacturer's instructions. qPCR analysis targeting genes associated with pan-adipocytes, adipogenesis, and lipogenesis was conducted using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..

    Article Title: Based on metabolome and transcriptome analysis, differences in organic acids of ‘Korla Xiangli’ fruit from different male parent
    Article Snippet: .. Gene-specific primers for the selected differentially expressed genes are listed in . qRT-PCR was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme). ..

    Article Title: Dynamics of severity-associated immune remodeling by granulocytes and macrophages in acute lung injury
    Article Snippet: Subsequently, RNA was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Organelle-specific hydrogen sulfide metabolism governs redox homeostasis to regulate plant autophagy and cadmium stress resilience
    Article Snippet: Specific primers for each gene were designed using Vector NTI Advance 10 software ( ). .. Real-time PCR was performed using iTaq universal SYBR qPCR Green Supermix (Bio-Rad), and the signals were detected on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) according to the manufacturer's instructions. ..

    SYBR Green Assay:

    Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology
    Article Snippet: For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the iScript Select cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions. .. Real-time qPCR was performed in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using specific primers ( ) and the iQ SYBR Green Mix (Bio-Rad), according to the manufacturer’s protocol. ..

    Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization
    Article Snippet: Total RNA was extracted using TRIpure RNA Isolation Reagent (TransGen Biotech) and reverse-transcribed using the 5× All-in-One RT Master Mix (ABM) according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT–PCR) was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using SYBR Green PCR Master Mix (ABclonal). ..

    Article Title: Dynamics of severity-associated immune remodeling by granulocytes and macrophages in acute lung injury
    Article Snippet: Subsequently, RNA was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (Takara) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Control:

    Article Title: Real-time PCR to target Hoodia in herbal supplements: a tool for conservation and trade regulation
    Article Snippet: .. All real-time PCR runs were conducted using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, USA) and included a No Template Control (NTC). ..

    Polymerase Chain Reaction:

    Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization
    Article Snippet: Total RNA was extracted using TRIpure RNA Isolation Reagent (TransGen Biotech) and reverse-transcribed using the 5× All-in-One RT Master Mix (ABM) according to the manufacturer’s instructions. .. Quantitative real-time PCR (qRT–PCR) was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using SYBR Green PCR Master Mix (ABclonal). ..

    Reverse Transcription:

    Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming
    Article Snippet: After removing the culture supernatant, total RNA was extracted using TRIzol reagent (Takara, 9109). .. Reverse transcription was performed according to the manufacturer's instructions. qPCR analysis targeting genes associated with pan-adipocytes, adipogenesis, and lipogenesis was conducted using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..



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